Antifungal Test AATCC Method 30
A standard antifungal test procedure is used here, AATCC Method 30. The material being tested is 100% cotton, treated with Ultra-Fresh. The fungus used to test it is Aspergillus niger, commonly found in the environment.
The test for antifungal properties starts out with a Petri plate carrying a layer of agar, containing nutrients that will support fungal growth. The microbiologist places a small piece of the sample on its surface. (Image 1)
A solution containing spores of Aspergillus niger is dropped onto the Petri plate and sample. (Image 2)
![]() Image 1 - click to enlarge |
![]() Image 2 - click to enlarge |
The plate is placed in an incubator, set at 28ºC, for 7 days. Fungi grow more slowly than bacteria; these conditions encourage optimal growth.
The following week, the microbiologist removes the plate from the incubator and assesses the situation.
In the right picture is an Ultra-Fresh treated sample (Image 4), the other, shown for the sake of comparison, is untreated (Image 3). This particular fungus causes mildew, which shows on the plates as a brown fuzz.
The sample treated with Ultra-Fresh (Image 4) has no fungus growing on it, or even close to it. The untreated sample, however, has done nothing to slow down the growth of the fungus.
Untreated![]() Image 3 - click to enlarge |
Treated![]() Image 4 - click to enlarge |










